Optimization of PCR Conditions for Detection of Human Brucellosis from Human Serum Samples
نویسندگان
چکیده
منابع مشابه
Optimization of PCR-ELISA in Detection of Human Cytomegalovirus Infection
Abstract Background and Objective: Human Cytomegalovirus (CMV) is an important cause of congenital viral infection that can lead to serious diseases and complications in infants. Application of rapid, sensitive, and specific HCMV detection methods is necessary for congenital infection detection. We aimed to optimize the use of PCR and ELISA for detection of HCMV in infants. Material and Methods...
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Polymerase chain reaction (PCR) is a rapid and simple technique with high sensitivity and specificity. Inthe recent years, PCR has been used for rapid detection of viral nucleic acids, such as Humancytomegalovirus (HCMV), whereas, PCR optimization is an important task to be done, especially beforeit’s diagnostic application. Annealing temperature, ion concentration (especially...
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A quantitative TaqMn Real-Time PCR assay was developed and its diagnostic value on human serum and livestock samples were evaluated. Brucella species could be distributed through communities as a biological agent. Rapid detection of biological threat agents is critical for timely therapeutic administration. Quantitative real-time PCR provides a rapid, sensitive and specific tool for molecular i...
متن کاملPCR assay for diagnosis of human brucellosis.
We have read with interest the recent article by Morata et al. reporting their study of two of the possible factors which can interfere with specific DNA amplification in a peripheralblood PCR assay used for the diagnosis of human brucellosis (2). The study deals with the optimization of a method described in a previous work by these authors (4), in which they report a sensitivity of 100% and a...
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Background and Aims: Influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. A variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. Methods: Nasal and pharyngeal samples taken from patients were inoculated into Madin-Darby canine kidney (MOCK) cells and embryonated chicken eggs (ECEs). The culture media was a...
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ژورنال
عنوان ژورنال: Research Journal of Microbiology
سال: 2008
ISSN: 1816-4935
DOI: 10.3923/jm.2008.352.358